Composite
Part:BBa_K3624007:Design
Designed by: Emily Hagens Group: iGEM20_Lethbridge (2020-10-26)
Tu-AMP2: Antifungal peptide.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 85
Illegal XbaI site found at 100
Illegal SpeI site found at 172
Illegal PstI site found at 186 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 85
Illegal SpeI site found at 172
Illegal PstI site found at 186
Illegal NotI site found at 91
Illegal NotI site found at 179 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 85
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 85
Illegal suffix found in sequence at 172 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 85
Illegal XbaI site found at 100
Illegal SpeI site found at 172
Illegal PstI site found at 186 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The amino acid sequence was codon-optimized.
Source
Tu-AMP2 comes from Tulip Bulbs (Tulipa gesneriana).
References
Fujimura, M., Ideguchi, M., Minami, Y., Watanabe, K., & Tadera, K. (2004). Purification, Characterization, and Sequencing of Novel Antimicrobial Peptides,Tu-AMP 1 andTu-AMP 2, from Bulbs of Tulip (Tulipa gesnerianaL.). Bioscience, Biotechnology, and Biochemistry, 68(3), 571-577. doi:10.1271/bbb.68.571